Lead Research Associate & Manager-Human Stem Cell & Genome Engineering Center
Location: Los Angeles/CA/USA
Required Education: High School
Internal Number: 21028
Under the direct supervision of the director of Human Pluripotent Stem Cell and Genome Engineering Lab, you will serve as a technical Subject Matter Expert (SME) to oversee the induced pluripotent stem cells (iPSCs) generation activities of the lab. In this role, your responsibilities include cell culture of mammalian cell lines including human primary somatic cells and human pluripotent stem cells, numerous reprogramming experiments from somatic cells to induced pluripotent stem cells, the characterization of resulting induced pluripotent stem cell lines and of the reprogramming processes, banking and organization of cell lines, the characterization (quality control) of resulting iPSC lines with diverse biochemical and molecular methods including genomics approaches such as RNA-seq and whole genome sequencing and CRISPR.
You will be required to coordinate and work together as a team with other employees of the core lab. The position will be highly cross-functional and will build and maintain effective collaborations with UCLA researchers and will oversee technical staff in the core. You will work with the lab personnel on other tasks as needed including ordering, distributing research materials, administration of projects and budgeting. This role will report to the director of Human Pluripotent Stem Cell and Genome Engineering Lab.
5+ years’ experience in reprogramming human primary somatic cells such as fibroblasts or blood samples to iPSCs without the use of antibiotic
5+ years’ experience in culturing iPSCs and human embryonic stem cells Flow cytometry
Previous research experience on CRISPR targeting & library screening and capacity to develop effective gene-editing tools & publications are highly preferred'
Routine passaging, freezing/expanding of stocks of iPSCs
Skilled with standard techniques in molecular biology, cell biology, biochemistry, genomics approaches and bioinformatics, including molecular cloning. The ability to troubleshoot related protocols.
Ability to establish a characterization workflow for iPSCs and somatic cell preparations
Experience with automation and screening
Experience with genomics approaches
Experience with interfacing with clients
Ability to establish new laboratory procedures and protocols successfully
Knowledge of lab safety including use of biohazardous and chemical materials and waste.
UCLA is an Equal Opportunity/Affirmative Action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or protected Veteran status.
At UCLA Health, you can help heal humankind, one patient at a time by improving health, alleviating suffering and delivering acts of kindness. As you do, you’ll achieve great things in your life and your career. We’re a world-class health organization with four hospitals consistently recognized among the nation’s very best as well as an internationally-renowned medical school, primary and specialty care clinics and much more. Within our dynamic, innovative and growing organization, you’ll find exceptional opportunities to make the most of your abilities in a supportive, empowering and inclusive environment. If you embrace our values of Integrity, Compassion, Respect, Teamwork, Excellence and Discovery we invite you to see all you can accomplish at UCLA Health.